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Study Explores How Different Marijuana Extracts Kill Types Of Cancer Cells

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Marijuana extracts can impair the survival of certain types of cancer cells and inhibit their spread, according to a recent study. But the effects of those extracts vary significant based on their specific chemical makeup.

Researchers found that treating cancer cells with isolated ingredients in cannabis, such as THC alone, does not appear to be especially effective—but full cannabis extracts showed more promise. However, with the plant containing hundreds of compounds that appear in different concentrations across strains and preparations, researchers had their work cut out for them in investigating how various cannabinoid combinations treated different types of cancer cells.

The team tested the antitumor effects of 12 whole cannabis extracts on 12 human cancer cell lines in order to “determine whether whole cannabis preparations with specific phytocannabinoid profiles could be advantageous as therapy for certain cancer sub-types.” The findings were published in the journal Oncotarget.

“Our results show that specific cannabis extracts impaired the survival and proliferation of cancer cell lines as well as induced apoptosis.”

Each cell sample was treated with a marijuana extract in increasing doses (2-10 µg/ml) over the course of 24 hours. There were five preparations of cannabis that proved especially potent for a wide range of cancer types but, in general, the study shows there’s significant variability in effectiveness for different cancer types—even when the cancers originated in the same organ.

Via Oncotarget.

For example, two distinct forms of prostate cancer cells were found to be most sensitive to entirely different marijuana extracts.

The cannabis preparations also ranged widely in their effectiveness in preventing the proliferation of cancer cells. When applied to multiplying cells, there were three extracts that reduced the growths to 37-51 percent of their original size, compared to 68 percent for the control group. But there were other extracts that failed to reduce the spread in a statistically significant manner.

Some commonalities shared among the most potent cannabis extracts include a high concentration of THC and large amounts of phytocannabinoids in their decarboxylated form.

“Taken as a whole, we concluded that medical cannabis does not consist of a single therapeutic agent but rather a heterogeneous array of treatments,” the researchers wrote. “We propose that the fate of specific cancer cells following cannabis extract application is dependent upon the synergistic effects of its phytocannabinoid composition, concentration applied, along with the cell specific characteristics (e.g. cannabimimetic receptor expression).”

“This study demonstrates the anti-cancer activity of various whole cannabis extracts on a set of human cancer cell lines.”

The study concluded that “cannabis extracts were very potent in producing cell death and some of these extracts were of [THC]-rich type” and that, as previous studies have indicated, “using whole cannabis extracts is more effective in inducing cancer cell death than applying pure [THC] on the studied cells lines.”

“Furthermore, not all [THC]-rich extracts produce the same effects when applied at the same concentrations on a specific cancer cell line,” the study authors wrote. “These findings indicate that compounds other than [THC] in these extracts might act together in a polypharmacology way and determine the extract efficacy as antitumor agents.”

Interestingly, the researchers also theorized that the the “presence or absence of [cannabinoid receptors] in the tested cell lines may explain the differential potency of the extracts towards reducing cell survival.”

The team called for further research into the “specific properties and mechanisms of cancer cell insensitivity to cannabis extract effects.”

“We hope that this study will lay the groundwork for future preclinical studies and randomized controlled clinical trials in order to provide evidence for effective cannabis treatments for many cancer subtypes,” they concluded.

Recreational Marijuana Store Customers Consume To Help Pain And Sleep, Study Finds

Photo courtesy of Evan Johnson.

Marijuana Moment is made possible with support from readers. If you rely on our cannabis advocacy journalism to stay informed, please consider a monthly Patreon pledge.

Kyle Jaeger is Marijuana Moment's Los Angeles-based associate editor. His work has also appeared in High Times, VICE and attn.

Politics

Federally Funded Journal Exposes How Marijuana Prohibition Puts Consumers At Risk

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Federal dollars are supporting the publication of a recent report that highlights how marijuana prohibition puts cannabis consumers at risk due to the resulting lack of guidance on safety standards from regulators.

The article was published this week in Environmental Health Perspectives, an “an open-access journal published with support from” the National Institutes of Health (NIH). It looked at issues related to regulating cannabis quality, which includes ensuring that the products don’t contain dangerous contaminants such as metals, pesticides and microbes.

“At the federal level in the United States, cannabis is still considered an illegal drug,” the piece notes. “As a result, neither the Food and Drug Administration (FDA) nor the Environmental Protection Agency (EPA) has provided any guidance on how to regulate contaminants or on which cannabis-related exposures can be considered safe.”

“States have had to determine on their own how to protect millions of cannabis users, and they have come up with widely varying responses,” the report states. “The result is an uncertain and occasionally incoherent regulatory landscape.”

The use of butane to extract marijuana concentrates, the prevalence of microbial contamination and high concentrations of metals are all concerns that federal agencies like the FDA and EPA would presumably address—if cannabis wasn’t a federally banned substance.

There’s at least one recent, relevant example on the issue: EPA announced on Wednesday that it will be approving pesticide applications for hemp, which was federally legalized through the Farm Bill late last year, but such tools will not be approved for marijuana because of its status under federal law.

But as it stands, such regulations are made and enforced at the state-level, meaning there’s a lack of consistency across legal marijuana programs.

“States have become experts at taxing and controlling this industry, and public health and safety has generally been a secondary or even-later-down-the-line consideration,” Ben Gelt, board chair of the Cannabis Certification Council, was quoted as saying in the report. “I think that is shifting, to some degree. I think that these issues are going to inevitably bubble up.”

Considering that instances of contamination have been reported in legalized states, it’s within reason to assume that cannabis consumers in non-legal states face an even greater set of risks given the complete lack of quality control standards.

“No state has it right, and there’s still a long way to go, and there’s still a lot of research that needs to be done,” Gelt said. “All of the states have significant gaps in their policies when it comes to testing and ensuring product quality and quality assurance. It just depends on what state you’re in where the gap is.”

While Environmental Health Perspectives receives support from federal government sources, an article’s appearance in the publication “does not indicate that the National Institute of Environmental Health Sciences condones, endorses, approves, or recommends the use of any products, services, materials, methodology, or policies stated therein,” according to a disclaimer on the journal’s website.

“Conclusions and opinions are those of the individual authors and advertisers only and do not necessarily reflect the policies or views of Environmental Health Perspectives or the National Institute of Environmental Health Sciences,” the notice states.

Nonetheless, the irony of federal dollars being spent to circulate a report hosted on a dot gov website that highlights the public health harms of ongoing federal marijuana prohibition was not lost on NORML Political Director Justin Strekal.

“The flat Earth mentality of continuing to deny the fact that a sizable percentage of the public consumes cannabis is hurting our ability to derive evidence-based best practices that put people’s health first,” he said. “It is the height of absurdity that public resources were used to compile a report that essentially states that the government is helpless because they have chained their hands to their sides as a result of prohibition. We demand regulation, not incarceration.”

“When the Congress chooses to get serious about putting public safety ahead of political expediency, they will move one of the various proposals to remove marijuana from the Controlled Substances Act to a vote,” Strekal said.

Marijuana Flower Offers More Pain Relief Than Other Cannabis Products, Study Finds

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Marijuana Flower Offers More Pain Relief Than Other Cannabis Products, Study Finds

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Among the overwhelming variety of cannabis products available on the market today, the most effective for pain relief appears to be whole dried marijuana flower and products high in THC, a new study finds.

“Whole cannabis flower was associated with greater pain relief than were other types of products, and higher tetrahydrocannabinol (THC) levels were the strongest predictors of analgesia and side effects prevalence across the five pain categories,” researchers from the University of New Mexico wrote. “In contrast, cannabidiol (CBD) levels generally were not associated with pain relief except for a negative association between CBD and relief from gastrointestinal and non-specified pain.”

Using data from a mobile app that aims to educate users about cannabis products and help them track their experiences, the researchers found that most people who reported self-medicating with marijuana have short-term, yet significant, relief from pain. “In our sample,” they write, “we observed an average pain reduction of roughly 3 points on a standard 0 to 10 visual analogue pain scale, consistent with its application as a mid-level analgesic.”

The findings, published in Complementary Therapies in Medicine in late July, are the latest in a robust body of scientific literature that shows marijuana can help people with different kinds of pain.

The study’s goal was to gauge how the severity of pain changed and what side effects were experienced after cannabis consumption, and whether these effects differed by product. Researchers used information gleaned from Releaf App, a mobile software program developed by three of the study’s authors and released in 2016. The app allows users to monitor their symptoms before, during and after consuming cannabis, thus helping them to understand the differences between products and delivery methods.

The study—which calls the Releaf data set “the largest database of real-time cannabis administration sessions in the U.S”—analyzed 20,513 cannabis sessions recorded in the app by 2,987 people between June 6, 2016 and October 24, 2018.

“Perhaps the most surprising result,” lead author Xiaoxue Li said in a statement, “is just how widespread relief was with symptom relief reported in about 95 percent of cannabis administration sessions and across a wide variety of different types of pain.”

“The results suggest that cannabis flower with moderate to high levels of tetrahydrocannabinol is an effective mid-level analgesic.”

On average, users reported their starting pain to be 5.87 on a scale of 1 to 10. After consuming marijuana, that number fell to 2.77—a decrease of 3.1 points.

“Among the limited number of product characteristics that are typically made available to consumers, we found that consumption of whole, natural Cannabis flower was associated with greater anesthetic potential than were most other types of products,” the authors wrote.

The study also found:

  • Patients whose cannabis sessions involved flower reported similar pain relief as those using concentrates and topicals. Edibles, pills and tinctures, however, offered less relief than flower.
  • Concentrates were found to be associated with more negative side effects, which the researchers reasoned could be because of solvents and other additives, as well as the removal of most terpenoids, terpenes and flavonoids.
  • Products labeled as hybrid strains were more effective at relieving pain than those labeled indica or sativa.
  • Combustion method didn’t affect pain.
  • Higher THC levels offered more pain relief, while higher CBD levels did not.
  • Patients with back, joint or muscle pain, headache or migraine and non-specified pain saw more relief with high-THC products.
  • Patients with gastrointestinal/abdominal-related pain found more relief with lower levels of THC.

As for other reactions, patients were more likely to report positive effects than negative effects: they cited dry mouth and feeling foggy as the most common negative ones, while feeling relaxed and peaceful were frequently reported as the most positive ones. Additionally, while CBD levels didn’t impact pain much, the cannabinoid did appear to decrease the likelihood of having negative side effects.

“The current findings,” the study concludes, “show that self-directed medical cannabis treatment, especially among users of higher THC products, is associated with significant improvements in at least short-term pain relief, perhaps a major reason why cannabis has become one of the most widely used medications in the United States.”

In a statement, Jacob Vigil, another study author and UNM associate professor of psychology, said the reason why dried cannabis flower may be more effective for pain is because of its “numerous constituents that possess analgesic properties beyond THC, including terpenes and flavonoids.” These compounds probably work together to increase cannabis’ therapeutic effects, he said.

“Our results confirm that cannabis use is a relatively safe and effective medication for alleviating pain, and that is the most important message to learn from our results,” Vigil continued. “It can only benefit the public for people to be able to responsibly weigh the true risks and benefits of their pain medication choices.”

Study Reveals How Marijuana Components THC And CBD Affect Chronic Pain

Photo courtesy of WeedPornDaily.

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Science & Health

Federal Data Shows Youth Marijuana Use Isn’t Increasing Under Legalization

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Despite prohibitionists’ often-expressed fears that legalization would lead more young people to use marijuana, new federal data released on Tuesday shows no such trend.

Reports of past-month cannabis use among those 12-17 remained stable from 2017 to 2018—and they’re significantly lower than in the years prior to when the first states began legalizing for adult use.

That’s according to the 2018 National Survey on Drug Use and Health (NSDUH), an annual report produced by the Substance Abuse and Mental Health Services Administration (SAMHSA).

“The percentage of adolescents in 2018 who used marijuana in the past year was lower than the percentages in 2002 to 2004 and in 2009 to 2013, but it was similar to the percentages in 2005 to 2008 and in 2014 to 2017,” SAMHSA said.

Via SAMHSA.

Recent studies that have used NSDUH data and other sources also demonstrate that youth marijuana use is not increasing post-legalization. In fact, research published in JAMA Pediatrics in July found that states with recreational cannabis experience a decline in underage marijuana use, with the study authors stating that regulated markets appear to deter illicit use.

Indeed, on a national scale, the percentage of adolescents who reported using marijuana began declining at a greater rate in the years after states started implementing legal cannabis systems. In 2018, 12.5 percent of those 12-17 said they used cannabis in the last month, compared to 13.5 percent in 2012, according to the NSDUH results.

Colorado and Washington State became the first U.S. states to vote to legalize marijuana for recreational use in late 2012, with legal sales commencing in 2014.

Between 2002 and 2018, the highest rate of adolescent marijuana use took place in 2002, when 15.8 percent reported past-month consumption.

“The survey results suggest that marijuana use among youth has remained stable and low in recent years, even as more states legalize medical and adult use,” Sheila Vakharia, PhD, deputy director of the Drug Policy Alliance’s Department of Research and Academic Engagement, told Marijuana Moment. “Rather than encouraging increased use, it is possible that legalization has limited access and deterred youth use. We find that these results strengthen the case for legalization in the interest of public health and protecting our nation’s young people.”

What’s more, the report found that cases of cannabis use disorder declined again for the 12-17 group in 2018, marking the seventh year in a row that fewer young people seem to be misusing the substance.

Via SAMHSA.

Interestingly, these trends are developing even as people’s perceptions of the risks of casual marijuana consumption are dropping. That seems to contradict an argument from reform opponents who claimed that legalizing cannabis would normalize it in such a way that underage individuals would feel more emboldened to experiment with marijuana.

Via SAMHSA.

Overall, marijuana consumption increased across age groups by about one percentage point over the past year, the survey found, with the bulk of that rise being attributable to those over 26. Past-month cannabis use for that demographic increased from 12.2 percent in 2017 to 13.3 percent in 2018.

Marijuana Taxes Differ In Legalized States, Complicating Projections

This story was updated to include comment from the Drug Policy Alliance.

Photo courtesy of Philip Steffan.

Marijuana Moment is made possible with support from readers. If you rely on our cannabis advocacy journalism to stay informed, please consider a monthly Patreon pledge.
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